Structure of PAP-IgM FcK fusion protein with J-chain expressed in transgenic plant
- Authors
- Kang, Yangjoo; Kim, Deuk-Su; Kim, Kibum; Myung, Soon-Chul; Oh, Yoo Jin; Park, Sungsu; Hinterdorfer, P.; Ko, Kisung
- Issue Date
- Jan-2023
- Publisher
- De Gruyter Open Ltd
- Keywords
- Immunoglobulin; Plant Biotechnology; Protein Engineering; Transgene
- Citation
- Eurobiotech Journal, v.7, no.1, pp 87 - 95
- Pages
- 9
- Journal Title
- Eurobiotech Journal
- Volume
- 7
- Number
- 1
- Start Page
- 87
- End Page
- 95
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66660
- DOI
- 10.2478/ebtj-2023-0006
- ISSN
- 2564-615X
2564-615X
- Abstract
- Transgenic plants expressing immunoglobulin (Ig) M Fc-fused Prostate acid phosphatase (PAP) antigenic proteins (PAP-IgM FcK) and J-chain proteins were generated by Agrobacterium-mediated transformation. The Fc region was tagged with the ER retention motif (KDEL) to make PAP-IgM FcK. Two transgenic plants were crossed together to generate F1 expressing both PAP-IgM FcK and J-chain proteins (PAP-IgM FcK × J-chain). PCR and RT-PCR analyses confirmed the transgene insertion and mRNA transcription of PAP-IgM FcK and J-chain in leaf tissue of PAP-IgM FcK × J-chain F1 plant. Western blot confirmed the expression of PAP-IgM FcK × J-chain protein. Size exclusion (SEC)-high performance liquid chromatography (HPLC) and Bio-transmission electron microscope (TEM) analyses were performed to show the size and shape of the PAP- IgM FcK × J-chain fusion proteins. These results suggest that PAP-IgM FcK with J-chain can be produced in plant expression system with plant crossing. © 2023 Yangjoo Kang et al., published by Sciendo.
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