Immune Responses to Plant-Derived Recombinant Colorectal Cancer Glycoprotein EpCAM-FcK Fusion Protein in Miceopen access
- Authors
- Lim, Chae-Yeon; Kim, Deuk-Su; Kang, Yangjoo; Lee, Ye-Rin; Kim, Kibun; Kim, Do Sun; Kim, Moon-Soo; Ko, Kisung
- Issue Date
- Nov-2022
- Publisher
- Korean Society of Applied Pharmacology
- Keywords
- Colorectal cancer; EpCAM; Germinal center B cells; Transgenic plants
- Citation
- Biomolecules and Therapeutics, v.30, no.6, pp 546 - 552
- Pages
- 7
- Journal Title
- Biomolecules and Therapeutics
- Volume
- 30
- Number
- 6
- Start Page
- 546
- End Page
- 552
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66661
- DOI
- 10.4062/biomolther.2022.103
- ISSN
- 1976-9148
2005-4483
- Abstract
- Epidermal cell adhesion molecule (EpCAM) is a tumor-associated antigen (TAA), which has been considered as a cancer vaccine candidate. The EpCAM protein fused to the fragment crystallizable region of immunoglobulin G (IgG) tagged with KDEL endoplas-mic reticulum (ER) retention signal (EpCAM-FcK) has been successfully expressed in transgenic tobacco (Nicotiana tabacum cv. Xanthi) and purified from the plant leaf. In this study, we investigated the ability of the plant-derived EpCAM-FcK (EpCAM-FcKP) to elicit an immune response in vivo. The animal group injected with the EpCAM-FcKP showed a higher differentiated germinal center (GC) B cell population (~9%) compared with the animal group injected with the recombinant rhEpCAM-Fc chimera (EpCAM-FcM). The animal group injected with EpCAM-FcKP (~42%) had more differentiated T follicular helper cells (Tfh) than the animal group injected with EpCAM-FcM (~7%). This study demonstrated that the plant-derived EpCAM-FcK fusion antigenic protein induced a humoral immune response in mice. © 2022 The Korean Society of Applied Pharmacology.
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