Inhibition of tumor growth by plant-derived mAbopen access
- Authors
- Ko, K; Steplewski, Z; Glogowska, M; Koprowski, H
- Issue Date
- May-2005
- Publisher
- NATL ACAD SCIENCES
- Keywords
- endoplasmic reticulum; glycosylation; Lys-Asp-Glu-Leu; mammalian-derived mAb
- Citation
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.102, no.19, pp 7026 - 7030
- Pages
- 5
- Journal Title
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Volume
- 102
- Number
- 19
- Start Page
- 7026
- End Page
- 7030
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66692
- DOI
- 10.1073/pnas.0502533102
- ISSN
- 0027-8424
1091-6490
- Abstract
- The tumor-associated antigen EpCAM (GA733-2) is a highly expressed target on adenocarcinoma cells, as defined by murine mAb CO17-1A. We recently developed a transgenic plant system for the safe and inexpensive production of large quantities of mAb CO17-1A as a future source of clinical-grade protein. Although the glycosylation pattern of plant-derived mAb (mAb(P)) CO17-1A differs considerably from that of the mammalian-derived mAb (mAb(M)), we show here that the biological activity of both mAbs is quite similar. mAbP heavy and light chains assembled to bind the recombinant antigen GA733-2E and specifically bound to human SW948 colorectal carcinoma cells expressing the antigen GA733-2 to the same extent as mAb(M). mAb(P) was as effective as mAb(M) CO17-1A in inhibiting tumor growth of xenotransplanted SW948 cells in nude mice. These results suggest the promise of transgenic plants as a useful alternative way to produce full-size mAb for cancer immunotherapy.
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