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Production of Ser125-interleukin-2 by Site Directed Mutagenesis특정 위치 변이방법에 의한 Ser125-Interleukin-2의 생산
- Authors
- Kang, Seongman; Kwak, Juwon; Kwon, Jong Bum; Kim, Sung Wan; Lee, In Young; Lee, Sun Bok; Yun, Hye-Young; Hahm, Kyung-Soo; Han, Moon H; Na, Doe Sun
- Issue Date
- 1989
- Publisher
- 한국생화학회
- Citation
- Korean Biochemical Journal, v.22, no.3, pp 306 - 311
- Pages
- 6
- Journal Title
- Korean Biochemical Journal
- Volume
- 22
- Number
- 3
- Start Page
- 306
- End Page
- 311
- Abstract
- Human Interleukin-2 (IL-2) posessing a serine in place of cystein at the amino acid sequence position 125 has been produced in E. coli by site directed mutagenesis. The activity of purified Ser^(125)-IL-2 was more than 2.5 times higher than that of Cys^(125)-IL-2 as determined by the growth promoting effect on IL-2 dependent cell line. The yield of production of Ser^(125)-IL-2 was at least 1.5 times higher as compared to that of Cys^(125)-IL-2.
아미노산 서열 125번 위치의 아미노산인 시스테인 세린으로 치환한 Interleukin-2를 특정 위치 변이방법에 의하여 대장균을 이용하여 생산하였다. Ser^(125)-IL-2는 IL-2 의존성인 배양세포에 대하여 Cys^(125)-IL-2와 유사한 성장촉진효과를 나타내었다. Ser^(125)-IL-2의 생산 수율은 Cys^(125)-IL-2에 비하여 1.5배 이상 높았다.
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