Differential functional expression of clotrimazole-sensitive Ca2+-activated K+ current in Bal-17 and WEHI-231 murine B lymphocytes
- Authors
- Zheng, Haifeng; Ko, Jae Hong; Nam, Joo Hyun; Earm, Yung E; Kim, Sung Joon
- Issue Date
- Feb-2006
- Publisher
- 대한약리학회
- Keywords
- B lymphocyte; Potassium channel; Ca2+−activated K+ channel; Clotrimazol; WEHI-231; Bal-17
- Citation
- The Korean Journal of Physiology & Pharmacology, v.10, no.1, pp 19 - 24
- Pages
- 6
- Journal Title
- The Korean Journal of Physiology & Pharmacology
- Volume
- 10
- Number
- 1
- Start Page
- 19
- End Page
- 24
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66782
- ISSN
- 1226-4512
2093-3827
- Abstract
- The intermediate conductance Ca2+-activated K+ channels (SK4, IKCa1) are present in lymphocytes, and their membrane expression is upregulated by various immunological stimuli. In this study, the activity of SK4 was compared between Bal-17 and WEHI-231 cell lines which represent mature and immature stages of murine B lymphocytes, respectively. The whole-cell patch clamp with high-Ca2+ (0.81μM) KCl pipette solution revealed a voltage-independent K+ current that was blocked by clotrimazole (1 mM), an SK4 blocker. The expression of mRNAs for SK4 was confirmed in both Bal-17 and WEIH-231 cells. The density of clotrimazole-sensitive SK4 current was significantly larger in Bal-17 than WEHI-231 cells (- 11.4±3.1 Vs. - 5.7±1.15 pA/pF). Also, the chronic stimulation of B cell receptors (BCR) by BCR-ligation (anti-IgM Ab, 3 μg/ml, 8 ∼ 12h) significantly upregulated the amplitude of clotrimazole-sensitive current from -11.4±3.1 to -53.1±8.6 pA/pF in Bal-17 cells. In WEHI-231 cells, the effect of BCR-ligation was significantly small (-5.7±1.15 to -9.0±1.00 pA/pF). The differential expression and regulation by BCR-ligation might reflect functional changes in the maturation of B lymphocytes.
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