Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Sphingosine-1-Phosphate-Induced ERK Activation Protects Human Melanocytes from UVB-Induced Apoptosis

Authors
Kim, D.-S.Kim, Sook-YoungLee, Jai-EunKwon, Sun-BangJoo, Young-HyunYoun, Sang-WoongPark, Kyoung-Chan
Issue Date
Sep-2003
Publisher
대한약학회
Citation
Archives of Pharmacal Research, v.26, no.9, pp 739 - 746
Pages
8
Journal Title
Archives of Pharmacal Research
Volume
26
Number
9
Start Page
739
End Page
746
URI
https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66884
DOI
10.1007/BF02976685
ISSN
0253-6269
1976-3786
Abstract
Ultraviolet B (UVB) is known to induce apoptosis in human melanocytes. Here we show the cytoprotective effect of sphingosine-1-phosphate (S1P) against UVB-induced apoptosis. We also show that UVB-induced apoptosis of melanocytes is mediated by caspase-3 activation and poly(ADP-ribose) polymerase (PARP) cleavage, and that S1P prevents apoptosis by inhibiting this apoptotic pathway. We further investigated three major mitogen-activated protein (MAP) kinases after UVB irradiation. UVB gradually activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, while extracellular signal-regulated protein kinase (ERK) was inactivated transiently. Blocking of the p38 MAP kinase pathway using SB203580 promoted cell survival and inhibited the activation of caspase-3 and PARP cleavage. These results suggest that p38 MAP kinase activation may play an important role in the UVB-induced apoptosis of human melanocytes. To explain this cytoprotective effect, we next examined whether S1P could inhibit UVB-induced JNK and p38 MAP kinase activation. However, S1P was not found to have any influence on UVB-induced JNK or p38 MAP kinase activation. In contrast, S1P clearly stimulated the phosphorylation of ERK, and the specific inhibition of the ERK pathway using PD98059 abolished the cytoprotective effect of S1P. Based on these results, we conclude that the activation of p38 MAP kinase plays an important role in UVB-induced apoptosis, and that S1P may show its cytoprotective effect through ERK activation in human melanocytes.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Medicine > College of Medicine > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kim, Dong Seok photo

Kim, Dong Seok
의과대학 (의학부(기초))
Read more

Altmetrics

Total Views & Downloads

BROWSE