Superoxide Dismutase 투여 후 고농도 산소에 노출된 흰쥐 뇌에서의 Superoxide Dismutase 및 Catalase 활성도 변화에 관한 연구

Abstract

In the present study, the changes in the production of the superoxide radicals and activities of superoxide dismnutase(Superoxide: superoxide oxidoreductase, EC 1. I5. 1. 1, SOD) and catalase (Hydrogen peroxide: hydrogen peroxide oxidoreductase, EC 1. 11. 1. 6)in the rat brains that were exposed to 95% O_2 or air following the intratracheal administration of SOD were investigated. The results were summarized as follows: 1. The activity of Cu, Zn-SOD in the brains of rats exposed to 95% O_2 after SOD administration were two to twelve times higher than those of rats exposed to air after either SOD or saline administration. 2. The activities of Mn-SOD in the rats that were exposed to 95% 0_2 after SOD or saline administration wire significally higher than those in the control group (p<0.01, p<0.15). 3. In the brains of rats treated with SOD, the production of superoxide radicals appeared to be negligible or very low in comparison with those in the control groups. The production of superoxide radicals in the rats brains that were exposed to 95% 0_2 after saline administration decreased sequentially. 4. Most of homogenates prepared from the brains of rats exposed to 95% 0_2 after SOD or saline administration showed the catalase activity similar to those in the control group. 5. The activity of catalase in the experimental group of SOD administration decreased at 30 min. 2 hr., 6hr. and 1 day exposed to air in comparison with those of the control group (p<0.01, p<0.05). In conclusion, it seems likely that the intratracheal administration of SOD, on one hand, accelerates the activityof SOD in the brains of rats which were exposed to 95% O_2 and it suppresses the production of superoxide radical. Accordingly the superoxide dismutase could protect the brain effectively from the harmful effects following the exposure to hyperoxic state.