Adriamycin과 Dimethylbenzanthracene의 투여에 의한 Mouse간장에서의 산소 독성 효과

Abstract

We studied the changes of the activities of superoxide dismutase (EC 1. 15. 1. 1, SOD) and catalase (EC 1. 11. 1. 6) and the production of superoxide radical in mice liver after application of adriamycin (ADM) and dimethylbenzanthracene (DMBA). Mice were injected subcutneously with ADM (5 or 10 mg/kg body weight for 16 days) followed by topical application of DMBA in dose of 20 mg/kg once a week for 4 times. The results were summarized as follows: 1. In all experimental groups, the activities of Cu, Zn-SOD and catalase showed lower level than those in the controls (p<0.01). 2. The activities of Mn-SOD in 4 weeks of ADM 5 mg/kg group and 3,4 and 6 weeks of ADM 10 mg/kg groups appeared to be lower than t se in t e controls (p<0.05, P<0.01). 3. Production of superoxide radical in all experimental groups increased in comparison with its control value (p<0.01). In conclusion, it seems likely that ADM and DMBA could decrease the activities of SOD and catalase which are one of the factors in the liver defence mechanism for the oxygen toxicity and increase the superoxide radical production. Therefore, oxygen toxicity may be developed in mouse liver by ADM and DMBA.