Lipopolysaccharide와 dexamethasone의 처치가 마우스 신장내 프리라디칼 계에 미치는 영향

Abstract

Lipopolysaccharide (LPS) develops sepsis through various immune and inflammatory reactions to induce the generation of reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI) in several different organs. In this study, the effects of dexamethasone (DM) on the generation of ROI and RNI as well as the activities of enzymes removing ROI were examined in the kidney of mouse model of sepsis with LPS. LPS (2.5 mg/kg body weight) significantly (p < 0.01) enhanced nitric oxide generation (NO) in serum. The increased NO generation, however, was effectively (p < 0.01) blocked by pretreatment with DM (10 mg/kg). The activity of xanthine oxidase, a marker for generation of superoxide radical, was increased by LPS (p < 0.01), and the enhancement was attenuated by pretreatment with DM. The activities of Mn-superoxide dismutase and glutathione peroxidase, ROI removing enzymes, were not changed in mouse kidney by LPS and/or DM. However, the activities of Cu,Zn-superoxide dismutase and catalase were effectively reduced by LPS and/or DM (p < 0.01 or p < 0.05). These results demonstrated that the effects of dexamethasone (DM) and LPS on the generation of ROI and RNI as well as the activities of enzymes removing ROI may play a pathophysiologic role in the kidney injury of mouse model of sepsis.