Effects of amitriptyline and imipramine on superoxide generation, myeloperoxidase release, leukotriene B4 production and calcium mobilization in human neutrophilsAmitriptyline과Imipramine이 호중구에서의 Superoxide 생성, Myeloperoxidase 유리, Leukotriene B4 생성과 칼슘 동원에 나타내는 영향
- Authors
- Shin, Yong Kyoo; Lee, Chung Soo.; Lee, Kwang Soo
- Issue Date
- Apr-1995
- Publisher
- Eui-Hak Publishing and Printing Co.
- Keywords
- Neutrophils; Heat aggregated IgG; Calmo-dulin,Amitriptyline; Imipramine
- Citation
- Korean Journal of Pharmacology, v.31, no.1, pp 123 - 133
- Pages
- 11
- Journal Title
- Korean Journal of Pharmacology
- Volume
- 31
- Number
- 1
- Start Page
- 123
- End Page
- 133
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/67003
- DOI
- 10.4196/kjpp.1995.31.1.123
- ISSN
- 0377-9459
- Abstract
- A number of tricyclic antidepressants appear to have inhibitory action on calmodulin. Although amitriptyline, imipramine and doxepine have been shown to inhibit calcium uptake, oxidative phosphorylation and ATPase activities, effects of amitriptyline, imipramine and doxepine on functional responses of human neutrophils have not been elucidated. In this study, effects of amitriptyline, imipramine and doxepine on superoxide and hydrogen peroxide generation, myeloperoxidase release, leukotriene B4 formation and intracellular calcium level were investigated. Superoxide and hydrogen peroxide production in heat aggregated IgG-activated neutrophils were inhibited by amitriptyline, imipramine and doxepine. EDTA, EGTA, verapamil and bepredil inhibited heat aggregated IgG-induced superoxide production. Chlorpromazine, trifluoperazine, staurosporine and H-7 also inhibited it. PMA-induced superoxide production was inhibited by amitriptyline, imipramine, doxepine, chlorpromazine and H-7. Amitriptyline, imipramine, chlorpromazine and trifluoperazine inhibited the myeloperoxidase release by heat aggregated IgG. Productions of LTB4 and 5-HETE in heat aggregated IgG-activated neutrophils were inhibited by amitriptyline, imipramine and doxepine. In neutrophils, elevation of intracellular calcium induced by heat aggregated IgG was inhibited by amitriptyline, imipramine, doxepine, chlorpromazine and EGTA, while verapamil slightly inhibited increase of intracellular calcium and H-7 did not inhibit it. These results suggest that the inhibitory effect of amitriptyline, imipramine and doxepine on respiratory burst, myeloperoxidase release and LTB4 production in heat aggregated IgG-activated neutrophils appears to be ascribed to the inhibition of calcium mobilization, calmodulin and protein kinase C.
- Files in This Item
-
Go to Link
- Appears in
Collections - College of Medicine > College of Medicine > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/67003)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.