G-proteins are involved in riluzole inhibition of high voltage-activated calcium channels in rat dorsal root ganglion neurons
- Authors
- Huang, Chao-Sheng; Song, Jin-Ho; Nagata, Keiichi; Twombly, Dennis; Yeh, Jay Z.; Narahashi, Toshio
- Issue Date
- Jul-1997
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- G-protein; N-ethylmaleimide; GDP-beta-S; protein kinase A; high voltage-activated calcium channel; pertussis toxin; neuroprotective drug; riluzole
- Citation
- BRAIN RESEARCH, v.762, no.1-2, pp 235 - 239
- Pages
- 5
- Journal Title
- BRAIN RESEARCH
- Volume
- 762
- Number
- 1-2
- Start Page
- 235
- End Page
- 239
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/67048
- DOI
- 10.1016/S0006-8993(97)00491-5
- ISSN
- 0006-8993
1872-6240
- Abstract
- Effects of riluzole on high voltage-activated (HVA) calcium channels of rat dorsal root ganglion neurons were studied using the whole-cell patch-clamp technique. Riluzole at 30 mu M inhibited the HVA currents. The onset and offset of riluzole inhibitory effect were slow usually taking more than 3 min. Riluzole inhibition of the HVA currents was abolished and partially reduced by addition of 500 mu M GDP-beta-S and 1 mM N-ethylmaleimide, respectively, to the pipette solution. Pre-treatment with pertussis toxin or application of depolarizing pre-pulses did not affect riluzole's inhibitory effect on the HVA currents. Riluzole inhibition of the HVA currents was also blocked by internal application of 50 mu g/ml protein kinase A inhibitory peptide. It was concluded that pertussis toxin-insensitive G-proteins and protein kinase A may be involved in riluzole inhibition of the HVA currents. (C) 1997 Elsevier Science B.V.
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