Murine Siva-1 and Siva-2, alternate splice forms of the mouse Siva gene, both bind to CD27 but differentially transduce apoptosis
DC Field | Value | Language |
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dc.contributor.author | Yoon, Yoosik | - |
dc.contributor.author | Ao, Zhaohui | - |
dc.contributor.author | Cheng, Yuan | - |
dc.contributor.author | Schlossman, Stuart F. | - |
dc.contributor.author | Prasad K.V.S. | - |
dc.date.accessioned | 2023-07-06T02:40:50Z | - |
dc.date.available | 2023-07-06T02:40:50Z | - |
dc.date.issued | 1999-11 | - |
dc.identifier.issn | 0950-9232 | - |
dc.identifier.issn | 1476-5594 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/67070 | - |
dc.description.abstract | CD27, a member of the TNFR family known to provide essential co-stimulatory signals for T cell growth and B cell Ig synthesis, can also mediate cell death. Using the CD27 cytoplasmic tail as the bait in yeast two hybrid assay, me previously cloned human Siva, a pro-apoptotic molecule. Here me report the characterization of the mouse Siva gene as a 4 kb sequence containing 4 exons and 3 introns. RT-PCR has revealed the presence of two forms of mouse Siva mRNA, the longer full length form Siva-1 and the shorter Siva-2 lacking the sequence coded by exon 2. Immunoblotting with anti-Siva (human) antibodies clearly demonstrate the presence of both Siva-1 and Siva-2. Cotransfection experiments in 293T cells reveal that mouse CD27 receptor can interact with both forms of Siva. Although mouse Siva-1 can trigger apoptosis in Rat-1 cells and in some of the mouse cell lines in transient transfection experiments, similar to the observation made with human Siva, intriguingly its alternate splice form, Siva-2 appears to be much less toxic. It is therefore likely that Siva-2 could regulate the function of Siva-1. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | STOCKTON PRESS | - |
dc.title | Murine Siva-1 and Siva-2, alternate splice forms of the mouse Siva gene, both bind to CD27 but differentially transduce apoptosis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/sj.onc.1203144 | - |
dc.identifier.bibliographicCitation | ONCOGENE, v.18, no.50, pp 7174 - 7179 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000083901900025 | - |
dc.identifier.scopusid | 2-s2.0-0033604448 | - |
dc.citation.endPage | 7179 | - |
dc.citation.number | 50 | - |
dc.citation.startPage | 7174 | - |
dc.citation.title | ONCOGENE | - |
dc.citation.volume | 18 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Siva | - |
dc.subject.keywordAuthor | CD27 | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | alternate splice forms | - |
dc.subject.keywordAuthor | murine Siva gene | - |
dc.subject.keywordAuthor | TNFR family | - |
dc.subject.keywordPlus | NECROSIS-FACTOR RECEPTOR | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | DOMAIN-CONTAINING RECEPTOR | - |
dc.subject.keywordPlus | T-CELL ACTIVATION | - |
dc.subject.keywordPlus | RING FINGER PROTEIN | - |
dc.subject.keywordPlus | DEATH-DOMAIN | - |
dc.subject.keywordPlus | CYTOPLASMIC DOMAIN | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | KINASE RIP | - |
dc.subject.keywordPlus | FAMILY | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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