Murine Siva-1 and Siva-2, alternate splice forms of the mouse Siva gene, both bind to CD27 but differentially transduce apoptosisopen access
- Authors
- Yoon, Yoosik; Ao, Zhaohui; Cheng, Yuan; Schlossman, Stuart F.; Prasad K.V.S.
- Issue Date
- Nov-1999
- Publisher
- STOCKTON PRESS
- Keywords
- Siva; CD27; apoptosis; alternate splice forms; murine Siva gene; TNFR family
- Citation
- ONCOGENE, v.18, no.50, pp 7174 - 7179
- Pages
- 6
- Journal Title
- ONCOGENE
- Volume
- 18
- Number
- 50
- Start Page
- 7174
- End Page
- 7179
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/67070
- DOI
- 10.1038/sj.onc.1203144
- ISSN
- 0950-9232
1476-5594
- Abstract
- CD27, a member of the TNFR family known to provide essential co-stimulatory signals for T cell growth and B cell Ig synthesis, can also mediate cell death. Using the CD27 cytoplasmic tail as the bait in yeast two hybrid assay, me previously cloned human Siva, a pro-apoptotic molecule. Here me report the characterization of the mouse Siva gene as a 4 kb sequence containing 4 exons and 3 introns. RT-PCR has revealed the presence of two forms of mouse Siva mRNA, the longer full length form Siva-1 and the shorter Siva-2 lacking the sequence coded by exon 2. Immunoblotting with anti-Siva (human) antibodies clearly demonstrate the presence of both Siva-1 and Siva-2. Cotransfection experiments in 293T cells reveal that mouse CD27 receptor can interact with both forms of Siva. Although mouse Siva-1 can trigger apoptosis in Rat-1 cells and in some of the mouse cell lines in transient transfection experiments, similar to the observation made with human Siva, intriguingly its alternate splice form, Siva-2 appears to be much less toxic. It is therefore likely that Siva-2 could regulate the function of Siva-1.
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