Plant-derived PAP proteins fused to immunoglobulin A and M Fc domains induce anti-prostate cancer immune response in miceopen accessPlant-derived PAP proteins fused to immunoglobulin A and M Fc domains induce anti-prostate cancer immune response in mice
- Authors
- Kang, Yang Joo; Kim, Deuk-Su; Kim, Seyoung; Seo, Young-Jin; Ko, Kisung
- Issue Date
- Jul-2023
- Publisher
- NLM (Medline)
- Keywords
- IgA; IgM; Prostate acid phosphatase; Prostate cancer; Re-combinant protein
- Citation
- BMB reports, v.56, no.7, pp 392 - 397
- Pages
- 6
- Journal Title
- BMB reports
- Volume
- 56
- Number
- 7
- Start Page
- 392
- End Page
- 397
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/67821
- DOI
- 10.5483/bmbrep.2022-0207
- ISSN
- 1976-6696
1976-670X
- Abstract
- In this study, recombinant Fc-fused Prostate acid phosphatase (PAP) proteins were produced in transgenic plants. PAP was fused to immunoglobulin (Ig) A and M Fc domain (PAP-IgA Fc and PAP-IgM Fc), which were tagged to the ER retention sequence KDEL to generate PAP-IgA FcK and PAP-IgM FcK. Agrobacteriummediated transformation was performed to produce transgenic tobacco plants expressing four recombinant proteins. Genomic PCR and RT-PCR analyses confirmed the transgene insertion and mRNA transcription of PAP-IgA Fc, PAP-IgM Fc, PAP-IgA FcK, and PAP-IgM FcK in tobacco plant leaves. Western blot confirmed the expression of PAP-IgA Fc, PAP-IgM Fc, PAP-IgA FcK, and PAP-IgM FcK proteins. SEC-HPLC and Bio-TEM analyses were performed to confirm the size and shape of the plant-derived recombinant PAP-Fc fusion proteins. In mice experiments, the plant-derived IgA and IgM Fc fused proteins induced production of total IgGs including IgG1 against PAP. This result suggests that IgA and IgM Fc fusion can be applied to produce recombinant PAP proteins as a prostate cancer vaccine in plant expression system. [BMB Reports 2023; 56(7): 392-397].
- Files in This Item
-
- Appears in
Collections - College of Medicine > College of Medicine > 1. Journal Articles
- College of Natural Sciences > Department of Life Science > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.