Toxigenic diversity of Bacillus cereus isolated from fresh produce and effects of various factors on the growth and the cytotoxicity of B. cereus
- Authors
- Han, Areum; Yoon, Jae-Hyun; Choi, Yun-Sun; Bong, Yujin; Jung, Gyusuck; Moon, Sung-Kwon; Lee, Sun-Young
- Issue Date
- Jan-2024
- Publisher
- KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
- Keywords
- Bacillus cereus; Cytotoxicity; Emetic toxin; Enterotoxin; Fresh produce
- Citation
- FOOD SCIENCE AND BIOTECHNOLOGY, v.33, no.1, pp 219 - 229
- Pages
- 11
- Journal Title
- FOOD SCIENCE AND BIOTECHNOLOGY
- Volume
- 33
- Number
- 1
- Start Page
- 219
- End Page
- 229
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/68106
- DOI
- 10.1007/s10068-023-01330-0
- ISSN
- 1226-7708
2092-6456
- Abstract
- This study analyzed the virulence, growth characteristics, and cytotoxicity of Bacillus cereus strains isolated from fresh produce, including romaine lettuce, sesame leaf, tomato, and cucumber grown by different methods. Polymerase chain reaction (PCR) was used to assess the toxigenic potential, and the cytotoxicity of B. cereus was estimated using cell-free supernatant in HEp-2 cells. The study found that hblD was the predominant diarrheal enterotoxin in the 59 isolated B. cereus strains, followed by nheB and hblC. The optimal temperatures for growth ranged from 42 to 44 degrees C, with the highest growth rates and shortest lag times. Cytotoxicity varied greatly depending on abiotic factors, including NaCl, pH, and medium, and was not always correlated with cell population. The study highlights the importance of establishing control measures to prevent B. cereus intoxication in fresh vegetables.
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