Phytochemical constituents of Lactuca serriola leaves and their content analysis by HPLC-UVopen access
- Authors
- Kim, J.; Lee, H.-D.; Choi, J.; Lee, S.
- Issue Date
- 2022
- Publisher
- Korean Society for Applied Biological Chemistry
- Keywords
- High-performance liquid chromatography-ultraviolet; Lactuca serriola; Quantitative analysis
- Citation
- Journal of Applied Biological Chemistry, v.65, no.3, pp 153 - 158
- Pages
- 6
- Journal Title
- Journal of Applied Biological Chemistry
- Volume
- 65
- Number
- 3
- Start Page
- 153
- End Page
- 158
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/68157
- DOI
- 10.3839/jabc.2022.020
- ISSN
- 1976-0442
2234-7941
- Abstract
- This study aimed to identify the phytochemical constituents of Lactuca serriola leaves and perform quantitative analysis of the methanol (MeOH) extract of L. serriola, L. indica, L. raddeana, L. sativa, and L. triangulata. Six compounds were isolated from the MeOH extracts of L. serriola using open column chromatography and identified as protocatechuic acid (1), caffeic acid (2), cynaroside (3), apigenin 7-glucuronide (4), luteolin (5), and apigenin (6) using1H-,13C-nuclear magnetic resonance, and mass spectrometry. Quantitative analysis of the six compounds was performed on the MeOH extract of Lactuca species using high-performance liquid chromatography (HPLC) and an ultraviolet (UV). A reverse-phased column was used, and the UV absorbance was set to 280 nm. The contents of compounds 2 and 3 were the highest (1.58 and 2.64 mg/g ext., respectively) in L. serriola extracts. However, compounds 4 and 6 were higher (1.46 and 0.40 mg/g ext., respectively) in L. triangulata. These results provide quantitative data for the application of Lactuca species in the pharmaceutical and functional food industries. © The Korean Society for Applied Biological Chemistry 2022.
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