단백질 고생산 야생형 효모 균주 선별 및 무작위 돌연변이를 통한 단백질 함량 증대Selection of Wild Type Saccharomyces cerevisiae with High Protein Content and Improving its Protein Content via Random Mutagenesis
- Authors
- Jeon, Gi-Beom; Lee, Young-Oh; Joo, Bumjin; Chin, Young-wook; Kim, Sun-Ki
- Issue Date
- Aug-2023
- Publisher
- 한국산업식품공학회
- Keywords
- single-cell protein; Saccharomyces cerevisiae; random mutagenesis
- Citation
- 산업식품공학, v.27, no.3, pp 199 - 205
- Pages
- 7
- Journal Title
- 산업식품공학
- Volume
- 27
- Number
- 3
- Start Page
- 199
- End Page
- 205
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/68469
- DOI
- 10.13050/foodengprog.2023.27.3.199
- ISSN
- 1226-4768
2288-1247
- Abstract
- Protein can be provided by cultivating various microbes, which contain more than 30% protein content by cell dry weight. This study compared intracellular protein concentrations of various wild-type yeasts from different sources to select the best yeast strain with high protein concentration. Among them, Saccharomyces cerevisiae KCCM 34709, used for molasses fermentation, exhibited 4.1-fold higher protein concentration than a laboratory yeast strain, S. cerevisiae D452-2. In this study, an approach consisting of random mutagenesis coupled with the Bradford protein assay-based screening method was applied to enhance the S. cerevisiae KCCM 34709 protein content. Among 1,000 mutants, the #180 mutant strain produced 5,041±519 mg/L total amino acid in 48 h, which was 31% higher than the parental S. cerevisiae KCCM 34709 strain. These results demonstrate that the #180 mutant strain can be an attractive cell factory for animal-free protein production.
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