siRNAs to Knockdown Antiviral Chemokine-related Genes in FRhK-4 Cells
DC Field | Value | Language |
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dc.contributor.author | Park, E. | - |
dc.contributor.author | Yoo, Y. | - |
dc.contributor.author | Park, S. | - |
dc.contributor.author | Choi, C. | - |
dc.contributor.author | Yoon, Y. | - |
dc.date.accessioned | 2023-11-08T01:46:59Z | - |
dc.date.available | 2023-11-08T01:46:59Z | - |
dc.date.issued | 2023-05 | - |
dc.identifier.issn | 0362-028X | - |
dc.identifier.issn | 1944-9097 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/68520 | - |
dc.description.abstract | The objective of this study was to generate small interfering RNA (siRNA) to knockdown antiviral chemokine-related genes in fetal rhesus monkey kidney (FRhK-4) cells. We generated siRNA duplexes to suppress antiviral chemokines like CXCL10 and CCL4 in FRhK-4 cells by downregulating interferon regulatory factor (IRF) 3 and IRF7. Three siRNA duplexes (si-F-IRF3-1, si-F-IRF3-2, and si-F-IRF3-3) targeting IRF3, and one siRNA duplex (si-F-IRF7) targeting IRF7 were generated. A nontarget siRNA duplex was used as the negative control. The nontarget or target siRNA duplexes (si-F-IRF3-1, si-F-IRF3-2, si-F-IRF3-3, and si-F-IRF7) were transfected into FRhK-4 cells using transfection reagents, and they were then incubated at 37°C for 6 h with 5% CO2. After 6 h, the medium was removed, and fresh medium was added to each cell, and they were then incubated at 37°C for 48 h with 5% CO2. The transfected FRhK-4 cells were infected with hepatitis A virus (HAV) HM-175/18f (viral titer: 105 PFU/mL) and incubated at 37°C for 3 h with 5% CO2 for HAV propagation. The expression levels of chemokines, including CXCL10 and CCL4, under the regulation of IRF3 and IRF7 in the transfected FRhK-4 cells were measured using quantitative real-time polymerase chain reaction after 3 h of HAV infection. The results indicated that CXCL10 and CCL4 expression levels were decreased in FRhK-4 cells transfected with si-F-IRF3-1, si-F-IRF3-3, or si-F-IRF7 (p < 0.05) compared to those in the negative control. These results indicate that si-F-IRF3-1 and si-F-IRF3-3, and si-F-IRF7 successfully knocked down IRF3 and IRF7 in FRhK-4 cells, respectively and suppressed antiviral chemokines. These siRNAs could be used to suppress antiviral chemokines in FRhK-4 cells. © 2023 The Author(s) | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier B.V. | - |
dc.title | siRNAs to Knockdown Antiviral Chemokine-related Genes in FRhK-4 Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.jfp.2023.100076 | - |
dc.identifier.bibliographicCitation | Journal of Food Protection, v.86, no.5 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 001029587300001 | - |
dc.identifier.scopusid | 2-s2.0-85151410706 | - |
dc.citation.number | 5 | - |
dc.citation.title | Journal of Food Protection | - |
dc.citation.volume | 86 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Anti-viral responses | - |
dc.subject.keywordAuthor | Chemokines | - |
dc.subject.keywordAuthor | Hepatitis A virus | - |
dc.subject.keywordAuthor | Interferon regulatory factors | - |
dc.subject.keywordAuthor | siRNAs | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | GROWTH | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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