Combination of paper membrane-based filtration and ultrafiltration to enhance the detection of MNV, HAV, and HCoV from soil-rich post-washing water
DC Field | Value | Language |
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dc.contributor.author | Wang, Z. | - |
dc.contributor.author | Jung, S. | - |
dc.contributor.author | Yeo, D. | - |
dc.contributor.author | Woo, S. | - |
dc.contributor.author | Seo, Y. | - |
dc.contributor.author | Hossain, M.I. | - |
dc.contributor.author | Kwon, H. | - |
dc.contributor.author | Jeong, M.-I. | - |
dc.contributor.author | Choi, C. | - |
dc.date.accessioned | 2023-11-08T02:47:49Z | - |
dc.date.available | 2023-11-08T02:47:49Z | - |
dc.date.issued | 2022-12 | - |
dc.identifier.issn | 2589-0042 | - |
dc.identifier.issn | 2589-0042 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/68526 | - |
dc.description.abstract | Risk-assessing and controlling virus transmission from soil-rich post-washing water (PWW) are crucial during harvesting raw vegetables. However, viruses are normally difficult to concentrate because of their low concentrations and complex backgrounds. Here, ultrafiltration (UF), virus adsorption-elution (VIRADEL), and optimized paper filtration-coupled ultrafiltration (PFC-UF) methods were employed to evaluate the recovery of non-enveloped murine norovirus (MNV-1), hepatitis A virus (HAV), and enveloped human coronavirus 229E (HCoV-229E) from soil-rich PWW. Among the three methods, PFC-UF outperformed the other methods in the recovery of viruses from PWW with soil content. Under the highest soil condition with virus seeded at a titer of 102 plaque-forming unit (PFU) or TCID50, the PFC-UF method exhibited an exceedingly consistent recovery rate of 78.8 ± 13.3 (MNV-1) and 44.4 ± 25.2% (HAV). However, the recovery of enveloped HCoV-229E was inferior to non-enveloped viruses. Overall, PFC-UF provided a reliable method for recovering viruses in soil-rich PWW. © 2022 The Author(s) | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier Inc. | - |
dc.title | Combination of paper membrane-based filtration and ultrafiltration to enhance the detection of MNV, HAV, and HCoV from soil-rich post-washing water | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.isci.2022.105640 | - |
dc.identifier.bibliographicCitation | iScience, v.25, no.12 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000908364400007 | - |
dc.identifier.scopusid | 2-s2.0-85143538326 | - |
dc.citation.number | 12 | - |
dc.citation.title | iScience | - |
dc.citation.volume | 25 | - |
dc.type.docType | Article; Early Access | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Membrane system | - |
dc.subject.keywordAuthor | Virology | - |
dc.subject.keywordPlus | ADSORPTION-ELUTION METHOD | - |
dc.subject.keywordPlus | ENTERIC VIRUSES | - |
dc.subject.keywordPlus | FOODBORNE VIRUSES | - |
dc.subject.keywordPlus | RIVER WATER | - |
dc.subject.keywordPlus | CHAIN | - |
dc.subject.keywordPlus | NOROVIRUS | - |
dc.subject.keywordPlus | CONTAMINATION | - |
dc.subject.keywordPlus | ENVIRONMENT | - |
dc.subject.keywordPlus | RETENTION | - |
dc.subject.keywordPlus | RECOVERY | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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