Localization of swine hepatitis E virus in liver and extrahepatic tissues from naturally infected pigs by in situ hybridization
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, C | - |
dc.contributor.author | Chae, C | - |
dc.date.accessioned | 2023-12-01T08:43:35Z | - |
dc.date.available | 2023-12-01T08:43:35Z | - |
dc.date.issued | 2003-06 | - |
dc.identifier.issn | 0168-8278 | - |
dc.identifier.issn | 1600-0641 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/68749 | - |
dc.description.abstract | Background/Aims: The objective of this study was to detect and localize the swine hepatitis E virus (HEV) in the liver and extrahepatic tissues from 20 pigs naturally infected with swine HEV. Methods: cDNA probe 289 base pairs for swine HEV were generated by reverse transcription-polymerase chain reaction. In situ hybridization with a nonradioactive digoxigenin-labeled cDNA probe was used for the detection of swine HEV in formalin-fixed, paraffin-embedded tissues. Results: When liver tissues from the pigs naturally infected with swine HEV were hybridized with the nonradioactive digoxigenin-labeled cDNA probe, a strong signal was seen in hepatocytes and bile duct. Positive hybridization signals were also detected in small and large intestine, lymph node, tonsil, spleen, and kidney. Conclusions: Swine HEV was detected primarily in the hepatocytes. Swine HEV may also replicate in tissues other than the liver. In situ hybridization described in the present study has greatly facilitated the application of in situ hybridization procedures to the clinical setting, thus allowing for the diagnosis of swine HEV infection while preserving the morphology of the tissue. (C) 2003 European Association for the Study of the Liver. Published by Elsevier Science B.V. All rights reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Localization of swine hepatitis E virus in liver and extrahepatic tissues from naturally infected pigs by in situ hybridization | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/S0168-8278(03)00115-6 | - |
dc.identifier.bibliographicCitation | JOURNAL OF HEPATOLOGY, v.38, no.6, pp 827 - 832 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000183065200017 | - |
dc.identifier.scopusid | 2-s2.0-0037902223 | - |
dc.citation.endPage | 832 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 827 | - |
dc.citation.title | JOURNAL OF HEPATOLOGY | - |
dc.citation.volume | 38 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | hepatitis E virus | - |
dc.subject.keywordAuthor | in situ hybridization | - |
dc.subject.keywordAuthor | liver | - |
dc.subject.keywordAuthor | swine | - |
dc.subject.keywordPlus | NON-B HEPATITIS | - |
dc.subject.keywordPlus | PHYLOGENETIC ANALYSIS | - |
dc.subject.keywordPlus | MACAQUE HEPATOCYTES | - |
dc.subject.keywordPlus | UNITED-STATES | - |
dc.subject.keywordPlus | NON-A | - |
dc.subject.keywordPlus | TRANSMISSION | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordPlus | STRAIN | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordPlus | HUMANS | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.