In-situ hybridization for the detection of inflammatory cytokines (IL-1, TNF-α and IL-6) in pigs naturally infected with Actinobacillus pleuropneumoniae

Abstract

The detection and distribution of interleukin-1 (IL-1), tumour necrosis factor-alpha (TNF-alpha) and IL-6 were studied, by in-situ hybridization with a nonradioactive digoxigenin-labelled probe, in formalin-fixed paraffin wax-embedded lung tissue from 10 pigs naturally infected with Actinobacillus pleuropneumoniae. A strong hybridization signal for IL-1, TNF-alpha and IL-6 was detected in "streaming" degenerate alveolar leucocytes (the so-called "oat cells") bordering zones of coagulative necrosis, and a less intense signal was seen in the dense zone of degenerate cells in granulation tissue surrounding the necrotic areas. IL-1 expression was also prominent in scattered endothelial cells bordering zones of coagulative necrosis. Simultaneous expression of all three cytokines was always associated with pleuropneumonic lung lesions. Expression of inflammatory cytokines was minimal in non-lesional lung tissue of the infected pigs and in normal lung from control pigs. The results suggest that these cytokines play a crucial role in mediating and regulating inflammation through cells of several types in A. pleuropneumoniae infection. (C) 1999 Harcourt Publishers Ltd.