Enhanced transbuccal salmon calcitonin (sCT) delivery: Effect of chemical enhancers and electrical assistance on <i>in vitro</i> sCT buccal permeation
- Authors
- Oh, Dong-Ho; Chun, Kyeung-Hwa; Jeon, Sang-Ok; Kang, Jeong-Won; Lee, Sangkil
- Issue Date
- Oct-2011
- Publisher
- ELSEVIER
- Keywords
- Salmon calcitonin; Buccal delivery; Peptide delivery; Absorption enhancers; Iontophoresis; Electrical assistance
- Citation
- EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v.79, no.2, pp 357 - 363
- Pages
- 7
- Journal Title
- EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
- Volume
- 79
- Number
- 2
- Start Page
- 357
- End Page
- 363
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69420
- DOI
- 10.1016/j.ejpb.2011.05.010
- ISSN
- 0939-6411
1873-3441
- Abstract
- This study investigates the combined effect of absorption enhancers and electrical assistance on transbuccal salmon calcitonin (sCT) delivery, using fresh swine buccal tissue. We placed 2001U (40 mu g/mL) of each sCT formulation containing various concentrations of ethanol, N-acetyl-t-cysteine (NAC), and sodium deoxyglycocholate (SDGC) onto the donor part of a Franz diffusion cell. Then, 0.5 mA/cm(2) of fixed anodal current was applied alone or combined with chemical enhancers. The amount of permeated sCT was analyzed using an ELISA kit, and biophysical changes of the buccal mucosa were investigated using FT-IR spectroscopy, and hematoxylin-eosin staining methods were used to evaluate histological alteration of the buccal tissues. The flux (J(s)) of sCT increased with the addition of absorption enhancer groups, but it was significantly enhanced by the application of anodal iontophoresis (ITP). FT-IR study revealed that all groups caused an increase in lipid fluidity but only the groups containing SDGC showed statistically significant difference. Although the histological data of SDGC groups showed a possibility for tissue damage, the present enhancing methods appear to be safe. In conclusion, the combination of absorption enhancers and electrical assistance is a potential strategy for the enhancement of transbuccal sCT delivery. (C) 2011 Elsevier B.V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69420)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.