In silico design and fabrication of an SFI chip-based microspheroid culture system
- Authors
- Park, Soon-Jung; Jung, Taek-Hee; Kim, Jong Hyun; Lee, Kyoung-Yong; Kim, Jeongyun; Ju, Jongil; Moon, Sung-Hwan
- Issue Date
- May-2022
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- BIOMATERIALS SCIENCE, v.10, no.11, pp 2991 - 3005
- Pages
- 15
- Journal Title
- BIOMATERIALS SCIENCE
- Volume
- 10
- Number
- 11
- Start Page
- 2991
- End Page
- 3005
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69790
- DOI
- 10.1039/d2bm00250g
- ISSN
- 2047-4830
2047-4849
- Abstract
- The emergence of microfluidic devices and computational fluid dynamics (CFD) has propelled the need for next-generation biomimetic cell culture platforms that are flexible for monitoring and regulation. Therefore, this study evaluated a CFD application in an in silico-designed and spheroid-based flow integration 3D cell culture chip (SFI chip) to illustrate cell culture, drug screening, cytokine delivery, and differentiation of cells in a platform that partially recapitulates the natural environment. Our results show that a flow rate of 0.05 mL h(-1) or less induced no physical stress in the SFI chip (15 mm), and uniform cell spheroids (approximately 200 mu m) were formed across the platform. The cultured cells were tested in several experimental contexts (co-culture, drug screening, cytokine delivery, and differentiation), demonstrating the usefulness of computational simulation in expediting discovery and simple and effective means to scale the production of standardized cell spheroids cultured under dynamic and natural conditions. Advanced cell culture technologies can be used to accelerate research and discovery and the preclinical and clinical development of cell and cell-free therapies for urgent medical needs.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69790)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.