A Porous Membrane-Mediated Isolation of Mesenchymal Stem Cells from Human Embryonic Stem Cells
- Authors
- Hong, Ki-Sung; Bae, Daekyeong; Choi, Youngsok; Kang, Sun-Woong; Moon, Sung-Hwan; Lee, Hoon Taek; Chung, Hyung-Min
- Issue Date
- Mar-2015
- Publisher
- MARY ANN LIEBERT, INC
- Citation
- TISSUE ENGINEERING PART C-METHODS, v.21, no.3, pp 322 - 329
- Pages
- 8
- Journal Title
- TISSUE ENGINEERING PART C-METHODS
- Volume
- 21
- Number
- 3
- Start Page
- 322
- End Page
- 329
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69842
- DOI
- 10.1089/ten.tec.2014.0171
- ISSN
- 1937-3384
1937-3392
- Abstract
- Pluripotent human embryonic stem cells (hESCs) acquire mesenchymal characteristics during the epithelial-mesenchymal transition (EMT) process. Here, we report a simple and an efficient isolation method for mesenchymal stem cells (MSCs) from hESCs undergoing EMT using a commercialized porous membrane transwell culture insert. Suspension culture of hESC colonies results in the formation of embryoid bodies, which adhered on the upper compartment of 8 mu m porous membrane in the presence of EMG2-MV media. The population migrating through the permeable membrane to the lower compartment not only exhibited EMT markers but also expressed high levels of a panel of typical MSC surface antigen markers, and demonstrated multipotent differentiation capability. In addition, they have a prolonged proliferation capacity without characteristics and chromosomal changes. Furthermore, the isolated MSCs significantly enhanced cardiac functions in a rat model of myocardial infarction (MI) as measured by the left ventricle wall thickness (MI control, 32.9%+/- 3.2% vs. hESCs-MSCs, 38.7%+/- 2.4%), scar length (MI control, 46.1%+/- 2.5% vs. hESCs-MSCs, 41.8%+/- 1.3%), fibrosis area (MI control, 34.3%+/- 1.6% vs. hESCs-MSCs, 28.9%+/- 3.5%), and capillary density. Our findings demonstrate an ease with which hESCs-MSCs can be effectively isolated using the porous membrane, which overcomes the lack of availability of MSCs for therapeutic applications in various diseased animal models.
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