Ground-State Conditions Promote Robust Prdm14 Reactivation and Maintain an Active Dlk1-Dio3 Region during Reprogrammingopen access
- Authors
- Habib, Omer; Habib, Gizem; Moon, Sung-Hwan; Hong, Ki-Sung; Do, Jeong Tae; Choi, Youngsok; Chang, Sung Woon; Chung, Hyung-Min
- Issue Date
- Jan-2014
- Publisher
- KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
- Keywords
- 2i conditions; Dlk1-Dio3; induced pluripotent stem cells; Prdm14
- Citation
- MOLECULES AND CELLS, v.37, no.1, pp 31 - 35
- Pages
- 5
- Journal Title
- MOLECULES AND CELLS
- Volume
- 37
- Number
- 1
- Start Page
- 31
- End Page
- 35
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69846
- DOI
- 10.14348/molcells.2014.2212
- ISSN
- 1016-8478
0219-1032
- Abstract
- Induced pluripotent stem cells (iPSCs) are capable of unlimited self-renewal and can give rise to all three germ layers, thereby providing a new platform with which to study mammalian development and epigenetic reprogramming. However, iPSC generation may result in subtle epigenetic variations, such as the aberrant methylation of the Dlk1-Dio3 locus, among the clones, and this heterogeneity constitutes a major drawback to harnessing the full potential of iPSCs. Vitamin C has recently emerged as a safeguard to ensure the normal imprinting of the Dlk1-Dio3 locus during reprogramming. Here, we show that vitamin C exerts its effect in a manner that is independent of the reprogramming kinetics. Moreover, we demonstrate that reprogramming cells under 2i conditions leads to the early upregulation of Prdm14, which in turn results in a highly homogeneous population of authentic pluripotent colonies and prevents the abnormal silencing of the Dlk1-Dio3 locus.
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