Self-assembly of Au@AgNR along M13 framework: A SERS nanocarrier for bacterial detection and killing
- Authors
- Bi, Liyan; Zhang, Huangruici; Hu, Wenchao; Chen, Jiadong; Wu, Yixuan; Chen, Hao; Li, Bingqian; Zhang, Zhiyang; Choo, Jaebum; Chen, Lingxin
- Issue Date
- Oct-2023
- Publisher
- Elsevier Ltd
- Keywords
- Antibacterial efficiency; Bacteria; M13 phage; Surface-enhanced Raman scattering (SERS)
- Citation
- Biosensors and Bioelectronics, v.237
- Journal Title
- Biosensors and Bioelectronics
- Volume
- 237
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69924
- DOI
- 10.1016/j.bios.2023.115519
- ISSN
- 0956-5663
1873-4235
- Abstract
- Self-assembled functional nanomaterials with electromagnetic hot spots are crucial and highly desirable in surface-enhanced Raman scattering (SERS). Due to its versatile biological scaffold, the M13 phage has been employed to produce novel nano-building blocks and devices. In this study, we propose a novel M13 phage-based SERS nanocarrier, that utilizes the pVIII capsid in M13 to conjugate Au@Ag core-shell nanorod (Au@AgNR) with linker carboxy-PEG-thiol (M13-Au@AgNR) and the pIII capsid to specifically target Escherichia coli (E. coli). The M13-Au@AgNR@DTTC (3,3′- diethylthiocarbocyanine iodide) SERS probe was used to detect E. coli in a concentration range of 6 to 6 × 105 cfu/mL, achieving a limit of detection (LOD) of 0.5 cfu/mL. The proposed SERS platform was also tested in real samples, showing good recoveries (92%–114.3%) and a relative standard deviation (RSD) of 1.2%–4.7%. Furthermore, the system demonstrated high antibacterial efficiency against E. coli, approximately 90%, as measured by the standard plate-count method. The investigation provides an effective strategy for in vitro bacteria detection and inactivation. © 2023 Elsevier B.V.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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