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4C Analysis of EBV-Host DNA Interactome
- Authors
- Kim, K.-D.; Lieberman, P.M.
- Issue Date
- 2023
- Publisher
- Humana Press Inc.
- Keywords
- 4C-seq; EBNA1; EBV; Host; Interactome
- Citation
- Methods in Molecular Biology, v.2610, pp 99 - 107
- Pages
- 9
- Journal Title
- Methods in Molecular Biology
- Volume
- 2610
- Start Page
- 99
- End Page
- 107
- ISSN
- 1064-3745
- Abstract
- EBV persist as multicopy episomes in latently infected cells and alter transcriptional program of host systems. Knowledge of EBV tethering site helps us understand how EBV attaches to and regulates the host chromosome. Here, we introduce a step-by-step protocol for 4C-seq analysis, including cell fixation, 4C-DNA construction, and sequencing library preparation performed with EBV-positive Burkitt’s lymphoma cells. The method can be applied in a variety of studies and cell-types to identify target loci associated with bait positions, such as viral episomes. © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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