Identification of high affinity peptides for capturing norovirus capsid proteins

Abstract

Here, we present a platform where novel short and linear peptide motifs that enable binding to norovirus capsid proteins are selected by M13 phage display. The best peptide which recognizes recombinant norovirus 6H-P2 proteins has a sequence of 'QHIMHLPHINTL' and the apparent dissociation binding constants (Kd,app) of the selected peptides was found to be 185 nM of affinity. © The Royal Society of Chemistry.