An IF-FISH Approach for Covisualization of Gene Loci and Nuclear Architecture in Fission Yeast
- Authors
- Kim, Kyoung-Dong; Iwasaki, O.; Noma, K.
- Issue Date
- 2016
- Publisher
- ELSEVIER ACADEMIC PRESS INC
- Keywords
- FISH; Fission yeast; Gene positioning; Genome organization; IF; Nuclear architecture; Nuclear membrane; Nucleolus
- Citation
- ENZYMES OF EPIGENETICS, PT B, v.574, pp 167 - 180
- Pages
- 14
- Journal Title
- ENZYMES OF EPIGENETICS, PT B
- Volume
- 574
- Start Page
- 167
- End Page
- 180
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/70725
- DOI
- 10.1016/bs.mie.2016.04.003
- ISSN
- 0076-6879
1557-7988
- Abstract
- Recent genomic studies have revealed that chromosomal structures are formed by a hierarchy of organizing processes ranging from gene associations, including interactions among enhancers and promoters, to topologically associating domain formations. Gene associations identified by these studies can be characterized by microscopic analyses. Fission yeast is a model organism, in which gene associations have been broadly mapped across the genome, although many of those associations have not been further examined by cell biological approaches. To address the technically challenging process of the visualization of associating gene loci in the fission yeast nuclei, we provide, in detail, an IF-FISH procedure that allows for covisualizing both gene loci and nuclear structural markers such as the nuclear membrane and nucleolus.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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