Mesonia aestuariivivens sp. nov., isolated from a tidal flat

Abstract

A Gram-negative, aerobic, non-flagellated and ovoid or rod-shaped bacterial strain (JHPTF-M18(T)), which was isolated from a tidal flat sediment in Republic of Korea, was taxonomically characterized. Strain JHPTF-M18(T) grew optimally at 25 degrees C, at pH 7.0-7.5 and in the presence of 2.0-3.0% (w/v) NaCl. 16S rRNA gene sequence analysis showed that strain JHPTF-M18(T) forms a phylogenetic lineage within the radiation comprising type strains of Mesonia species. The 16S rRNA gene of strain JHPTF-M18(T) shared sequence similarities of 97.7% with that of type strain of M. mobilis and 92.5-96.8% with those of type strains of the other nine Mesonia species. The DNA G+C content was 33.1% based on its genomic sequence. AAI, ANI and dDDH values between strain JHPTF-M18(T) and the type strains of M. mobilis, M. hitae, M. oceanica, M. phycicola and M. algae were 72.1-83.7%, 73.1-79.7% and 18.5-22.8%, respectively. Strain JHPTF-M18(T) contained MK-6 as the predominant menaquinone and iso-C-15:0, iso-C-17:0 3-OH and summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c) as its major fatty acids. Major polar lipids of strain JHPTF-M18(T) were phosphatidylethanolamine and two unidentified lipids. Strain JHPTF-M18(T) was separated from recognized Mesonia species by its phenotypic properties together with the phylogenetic and genetic distinctiveness. Based on data presented in this study, strain JHPTF-M18(T) is considered to represent a novel species of the genus Mesonia. The name Mesonia aestuariivivens sp. nov. is proposed for JHPTF-M18(T) (=KACC 22185(T) = NBRC 115119(T)).