Bioconversion of citrus waste into mucic acid by xylose-fermenting Saccharomyces cerevisiae
- Authors
- Jeong, Deokyeol; Park, Sujeong; Evelina, Grace; Kim, Suhyeung; Park, Heeyoung; Lee, Je Min; Kim, Sun-Ki; Kim, In Jung; Oh, Eun Joong; Kim, Soo Rin
- Issue Date
- Feb-2024
- Publisher
- Elsevier Ltd
- Keywords
- CRISPR/Cas9; Delta-integration strategy; Fruit waste; meso-galactarate; Microbial bioconversion; Pectin-rich biomass
- Citation
- Bioresource Technology, v.393
- Journal Title
- Bioresource Technology
- Volume
- 393
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/71353
- DOI
- 10.1016/j.biortech.2023.130158
- ISSN
- 0960-8524
1873-2976
- Abstract
- Mucic acid holds promise as a platform chemical for bio-based nylon synthesis; however, its biological production encounters challenges including low yield and productivity. In this study, an efficient and high-yield method for mucic acid production was developed by employing genetically engineered Saccharomyces cerevisiae expressing the NAD+-dependent uronate dehydrogenase (udh) gene. To overcome the NAD+ dependency for the conversion of pectin to mucic acid, xylose was utilized as a co-substrate. Through optimization of the udh expression system, the engineered strain achieved a notable output, producing 20 g/L mucic acid with a highest reported productivity of 0.83 g/L-h and a theoretical yield of 0.18 g/g when processing pectin-containing citrus peel waste. These results suggest promising industrial applications for the biological production of mucic acid. Additionally, there is potential to establish a viable bioprocess by harnessing pectin-rich fruit waste alongside xylose-rich cellulosic biomass as raw materials. © 2023 Elsevier Ltd
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