Adipose tissue-derived exosomes alleviate particulate matter-induced inflammatory response and skin barrier damage in atopic dermatitis-like triple-cell modelopen access
- Authors
- Roh, Yoon Jin; Choi, Yong Hee; Shin, Sun Hye; Lee, Mi-Kyung; Won, Yu Jin; Lee, Jun Ho; Cho, Byong Seung; Park, Kui Young; Seo, Seong Jun
- Issue Date
- Jan-2024
- Publisher
- Public Library of Science
- Citation
- PLoS ONE, v.19, no.1
- Journal Title
- PLoS ONE
- Volume
- 19
- Number
- 1
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/71676
- DOI
- 10.1371/journal.pone.0292050
- ISSN
- 1932-6203
- Abstract
- Recently, particulate matter (PM) has been shown to exacerbate atopic dermatitis (AD) by inducing an inflammatory response. Meanwhile, several studies revealed that exosomes derived from adipose tissue-derived mesenchymal stem cells promote wound healing and alleviate inflammation via their regenerative and immunomodulatory capacities. Our study aimed to investigate the effects of human adipose tissue-derived mesenchymal stem cell-derived (ASC)-exosomes in PM-induced AD. An AD-like triple-cell model was established by treating human keratinocytes, dermal fibroblasts, and mast cells with polyinosinic:polycytidylic acid (Poly I:C) and interleukin 1 alpha (IL-1α). The effects of PM and ASC-exosomes on the expression of pro-inflammatory cytokines and skin barrier proteins were examined using quantitative real-time polymerase chain reaction, western blotting, and immunofluorescence. PM increased pro-inflammatory cytokines (IL-6, IL-1β, and IL-1α) and decreased the anti-inflammatory cytokine IL-10, while the mRNA expression of skin barrier proteins (loricrin and filaggrin) decreased. However, when the cells were treated with ASC-exosomes, the PM-induced effects on pro-inflammatory cytokines and skin barrier proteins were reversed. Our results confirmed that PM-induced inflammation and skin barrier damage were alleviated by ASC-exosomes in our AD-like triple-cell model. These data suggest that ASC-exosomes can serve as a therapeutic agent for PM-exacerbated AD. Copyright: © 2024 Roh et al.
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