Bilateral Effect of the Unilateral Corneal Nerve Cut on Both Ocular Surface and Lacrimal Glandopen access
- Authors
- Lee, Hyo Kyung; Kim, Kyoung Woo; Ryu, Jin Suk; Jeong, Hyun Jeong; Lee, Sang-Mok; Kim, Mee Kum
- Issue Date
- Jan-2019
- Publisher
- ASSOC RESEARCH VISION OPHTHALMOLOGY INC
- Keywords
- corneal nerve; dry eye; ocular surface; lacrimal gland; ocular immune privilege
- Citation
- INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, v.60, no.1, pp 430 - 441
- Pages
- 12
- Journal Title
- INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
- Volume
- 60
- Number
- 1
- Start Page
- 430
- End Page
- 441
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/71787
- DOI
- 10.1167/iovs.18-26051
- ISSN
- 0146-0404
1552-5783
- Abstract
- PURPOSE. This study investigated the effect of a unilateral cut of the corneal nerve on the bilateral ocular surface and tear secretory function. METHODS. Seven-week-old female BALB/c mice were divided into control and nerve-cutting (NC) groups (n= 60). The left cornea was partially incised with a 2-mm circular trephine through the upper half of the stromal layer. Lissamine green corneal staining and tear volume measurements were conducted, and corneal whole-mount staining using class III b-tubulin antibody was performed to assess corneal nerves. Flow cytometric analyses for dendritic cells (DCs), CD4b(+)/CD8(+) and regulatory T cells and ELISA for neuropeptides were performed. RESULTS. The grading of corneal staining increased in the NC group, while the tear volume decreased over the 4 weeks. The nerve density decreased in bilateral corneas over 2 weeks. At day 14, CD11b(+) or CD11c(+) DCs and the mature DCs expressing CD86 or MHCII increased in bilateral cornea/conjunctiva. At day 28, CD11c(+) CD86(hi), CD11c(+)MHCII(hi), Th17 and IFN-csecreting CD8(+) T cells highly increased in bilateral draining lymph nodes. CD4(+)CD25(hi)Foxp3(hi) and CD8(+)CD25(hi)Foxp3(hi) regulatory T cells notably increased in the spleen. In ELISA, neuropeptide Y, calcitonin gene-related peptide, and vasoactive intestinal peptide were generally suppressed in the extraorbital lacrimal glands at day14. CONCLUSIONS. The unilateral corneal nerve severing resulted in activation of the immune cells on the ocular surface and dysregulated lacrimal secretion bilaterally through the bidirectional neuronal signals. It suggests that the unilateral corneal nerve damage may alter immune homeostasis and mechanistically participate in the development of bilateral inflammatory disorders such as dry eye.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/71787)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.