Anti-Inflammatory Effects of the LK5 Herbal Complex on LPS- and IL-4/IL-13-Stimulated HaCaT Cells and a DNCB-Induced Animal Model of Atopic Dermatitis in BALB/c Miceopen access
- Authors
- Kim, Hyun-Jeong; Kim, So-Yeon; Bae, Ho Jung; Choi, Yu-Yeong; An, Ju-Yeon; Cho, Ye Eun; Cho, So-Young; Lee, Su-Jung; Lee, Sanghyun; Sin, MinSub; Yun, Young Min; Lee, Jong Ryul; Park, Se Jin
- Issue Date
- Jan-2024
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Keywords
- anti-inflammation; atopic dermatitis; itching; LK5 herbal complex; signal transducers and activators of transcription
- Citation
- Pharmaceutics, v.16, no.1
- Journal Title
- Pharmaceutics
- Volume
- 16
- Number
- 1
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/72804
- DOI
- 10.3390/pharmaceutics16010040
- ISSN
- 1999-4923
1999-4923
- Abstract
- Atopic dermatitis (AD) is a chronic inflammatory skin disease influenced by a complex interplay of genetic and environmental factors. The activation of the JAK-STAT pathway increases the expression of inflammatory cytokines such as IL-4 and IL-13, further deteriorating AD. Therefore, for the treatment of AD, the JAK-STAT pathway is emerging as a significant target, alongside inflammatory cytokines. This study investigates the potential therapeutic effects of a novel herbal complex, LK5, composed of Scutellaria baicalensis, Liriope platyphylla, Sophora flavescens, Dictammus dasycarpus, and Phellodendron schneider, known for their anti-inflammatory and immune-modulating properties. We examined the anti-inflammatory and anti-AD effects of the LK5 herbal complex in HaCaT cells stimulated by LPS and IL-4/IL-13, as well as in a mouse model of AD induced by DNCB. In HaCaT cells stimulated with LPS or IL-4/IL-13, the LK5 herbal complex demonstrated anti-inflammatory effects by inhibiting the expression of inflammatory cytokines including TNF-α, IL-6, and IL-1β, and downregulating the phosphorylation of STAT proteins. In a murine AD-like model induced by DNCB, administration of the LK5 herbal complex significantly ameliorated clinical symptoms, including dermatitis, ear thickness, and TEWL. Histological analysis revealed a reduction in epidermal thickness and mast cell infiltration. The LK5 herbal complex also inhibited pruritus induced by compound 48/80. Furthermore, the LK5 herbal complex treatment significantly decreased the levels of inflammatory cytokines such as TSLP, IL-6, and IgE in plasma and ear tissue of AD-induced mice. These findings suggest that the LK5 herbal complex may modulate the immune response and alleviate AD symptoms by inhibiting STAT pathways. © 2023 by the authors.
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