Synergistic Effect of Electrical and Biochemical Stimulation on Human iPSC-Derived Neural Differentiation in a Microfluidic Electrode Array Chip
- Authors
- Kim, Na Yeon; Choi, Yoon Young; Kim, Tae Hyeon; Ha, Jang Ho; Kim, Tae-Hyung; Kang, Taewook; Chung, Bong Geun
- Issue Date
- Mar-2024
- Publisher
- American Chemical Society
- Keywords
- biochemical stimulation; costimulation; electrical stimulation; human induced pluripotent stem cells (iPSCs); neural cell differentiation
- Citation
- ACS Applied Materials and Interfaces, v.16, no.13, pp 15730 - 15740
- Pages
- 11
- Journal Title
- ACS Applied Materials and Interfaces
- Volume
- 16
- Number
- 13
- Start Page
- 15730
- End Page
- 15740
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/73170
- DOI
- 10.1021/acsami.3c17108
- ISSN
- 1944-8244
1944-8252
- Abstract
- Neural differentiation is crucial for advancing our understanding of the nervous system and developing treatments for neurological disorders. The advanced methods and the ability to manipulate the alignment, proliferation, and differentiation of stem cells are essential for studying neuronal development and synaptic interactions. However, the utilization of human induced pluripotent stem cells (iPSCs) for disease modeling of neurodegenerative conditions may be constrained by the prolonged duration and uncontrolled cell differentiation required for functional neural cell differentiation. Here, we developed a microfluidic chip to enhance the differentiation and maturation of specific neural lineages by placing aligned microelectrodes on the glass surface to regulate the neural differentiation of human iPSCs. The utilization of electrical stimulation (ES) in conjunction with neurotrophic factors (NF) significantly enhanced the efficiency in generating functional neurons from human iPSCs. We also observed that the simultaneous application of NF and ES to human iPSCs promoted their differentiation and maturation into functional neurons while increasing synaptic interactions. Our research demonstrated the effect of combining NF and ES on human iPSC-derived neural differentiation. © 2024 American Chemical Society.
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