Enzymatic extraction of anthocyanins from the pomace of aronia (Aronia melanocarpa)
- Authors
- Kim, Jinman; Lee, Yejin; Suh, Hee-Jae; Lee, Hee-Seok; Lee, Chan
- Issue Date
- May-2024
- Publisher
- OXFORD UNIV PRESS
- Keywords
- anthocyanin pigments; aronia pomace; enzymatic extraction
- Citation
- Bioscience, biotechnology, and biochemistry, v.88, no.6, pp 639 - 647
- Pages
- 9
- Journal Title
- Bioscience, biotechnology, and biochemistry
- Volume
- 88
- Number
- 6
- Start Page
- 639
- End Page
- 647
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/73254
- DOI
- 10.1093/bbb/zbae037
- ISSN
- 0916-8451
1347-6947
- Abstract
- Efficient extraction of natural pigments is a key focus in enhancing the utilization of by-products for applications in the food industry. In this study, an enzymatic extraction method using Pectinex Ultra SP-L, Pectinex XXL, Novoshape, and Celluclast was used to investigate natural pigment production from the pomace of aronia, a commercially important plant. The method's performance was monitored using high-performance liquid chromatography with diode-array detection by measuring total and individual anthocyanin levels. Pectinex XXL (0.5%) yielded the highest total anthocyanin extraction (2 082.41 ± 85.69 mg/100 g) in the single enzyme treatment, followed by Pectinex Ultra SP-L (0.05%), Celluclast (0.01%), and Novoshape (0.1%). Combining Pectinex XXL (0.25%) with Celluclast (0.01%) increased the extraction ratio of total anthocyanins (2 323.04 ± 61.32 mg/100 g) by approximately 50.7% compared to that obtained using the solvent extraction method. This study demonstrated an effective enzymatic extraction method for application in the food industry. © The Author(s) 2024. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.
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