Ethanol Extracts of Cornus alba Improve Benign Prostatic Hyperplasia by Inhibiting Prostate Cell Proliferation through Modulating 5 Alpha-Reductase/Androgen Receptor Axis-Mediated Signalingopen access
- Authors
- Hwang, Byungdoo; Kim, Jongyeob; Park, Solbi; Chung, Hyun Joo; Kim, Hoon; Choi, Yung Hyun; Kim, Wun-Jae; Myung, Soon Chul; Jeong, Tae-Bin; Kim, Kyung-Mi; Jung, Jae-Chul; Lee, Min-Won; Kim, Jin Wook; Moon, Sung-Kwon
- Issue Date
- Apr-2024
- Publisher
- KOREAN SOC SEXUAL MEDICINE & ANDROLOGY
- Keywords
- BPH rat model; Benign prostatic hyperplasia; Ethanol extracts of; RWPE-1; WPMY-1
- Citation
- The world journal of men's health
- Journal Title
- The world journal of men's health
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/73465
- DOI
- 10.5534/wjmh.230200
- ISSN
- 2287-4208
2287-4690
- Abstract
- OBJECTIVE: The aim of this study was to investigate the efficacy of ethanol extracts of Cornus alba (ECA) against benign prostatic hyperplasia (BPH) in vitro and in vivo. METHODS: The prostate stromal cells (WPMY-1) and epithelial cells (RWPE-1) were used to examine the action mechanism of ECA in BPH in vitro. ECA efficacy was evaluated in vivo using a testosterone propionate (TP)-induced BPH rat model. RESULTS: Treatment with ECA inhibited the proliferation of prostate cells by inducing G1-phase cell cycle arrest through the regulation of positive and negative proteins. Treatment of prostate cells with ECA resulted in alterations in the mitogen-activated protein kinases and protein kinase B signaling pathways. The transcriptional binding activity of the NF-κB motif was suppressed in both ECA-treated prostate cells. In addition, treatment with ECA altered the level of BPH-associated axis markers (5α-reductase, fibroblast growth factor-2, androgen receptor, epidermal growth factor, Bcl-2, and Bax) in both cell lines. Finally, the administration of ECA attenuated the enlargement of prostatic tissues in the TP-induced BPH rat model, accompanied by histology, immunoblot, and serum dihydrotestosterone levels. CONCLUSIONS: These results demonstrated that ECA exerted beneficial effects on BPH both in vitro and in vivo and might provide valuable information in the development of preventive or therapeutic agents for improving BPH. Copyright © 2024 Korean Society for Sexual Medicine and Andrology.
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