Preadipocytes potentiate melanoma progression and M2 macrophage polarization in the tumor microenvironment
- Authors
- Jeon, Tae Jin; Kim, Ok-Hyeon; Kang, Hana; Lee, Hyun Jung
- Issue Date
- Aug-2024
- Publisher
- Elsevier B.V.
- Keywords
- Melanoma; miR-155; Preadipocytes; Tumor microenvironment
- Citation
- Biochemical and Biophysical Research Communications, v.721
- Journal Title
- Biochemical and Biophysical Research Communications
- Volume
- 721
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/74213
- DOI
- 10.1016/j.bbrc.2024.150129
- ISSN
- 0006-291X
1090-2104
- Abstract
- Melanoma, the deadliest skin cancer, originates from epidermal melanocytes. The influence of preadipocytes on melanoma is less understood. We co-cultured mouse melanoma B16 cells with 3T3L1 preadipocytes to form mixed spheroids and observed increased melanoma proliferation and growth compared to B16-only spheroids. Metastasis-related proteins YAP, TAZ, and PD-L1 levels were also higher in mixed spheroids. Treatment with exosome inhibitor GW4869 halted melanoma growth and reduced expression of these proteins, suggesting exosomal crosstalk between B16 and 3T3L1 cells. MiR-155 expression was significantly higher in mixed spheroids, and GW4869 reduced its levels. Additionally, co-culturing with Raw264.7 macrophage cells increased M2 markers IL-4 and CD206 in Raw264.7 cells, effects that were diminished by GW4869. These results indicate that preadipocytes may enhance melanoma progression and metastasis via exosomal interactions. © 2024 Elsevier Inc.
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