The quantification of HER2 and MYC gene fragments in cell-free plasma as putative biomarkers for gastric cancer diagnosis
- Authors
- Park, Kyoung Un; Lee, Hee Eun; Nam, Soo Kyung; Nam, Kyung Han; Park, Do Joong; Kim, Hyung-Ho; Kim, Woo Ho; Lee, Hye Seung
- Issue Date
- Jul-2014
- Publisher
- WALTER DE GRUYTER GMBH
- Keywords
- cell-free plasma; HER2; MYC; real-time PCR; stomach cancer
- Citation
- CLINICAL CHEMISTRY AND LABORATORY MEDICINE, v.52, no.7, pp 1033 - 1040
- Pages
- 8
- Journal Title
- CLINICAL CHEMISTRY AND LABORATORY MEDICINE
- Volume
- 52
- Number
- 7
- Start Page
- 1033
- End Page
- 1040
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/74785
- DOI
- 10.1515/cclm-2013-0988
- ISSN
- 1434-6621
1437-4331
- Abstract
- Background: This study aimed to investigate the significance of circulating HER2 and MYC gene fragments quantification in the diagnosis of gastric cancer. Methods: Levels of HER2 and MYC genes were evaluated by fluorescence in situ hybridization and real-time PCR in 81 gastric cancer tissues, and by real-time PCR in 36 gastritis tissues. Real-time PCR for HER2 and MYC products was also performed on 184 plasma samples from 81 gastric cancers, eight gastric adenomas, 63 gastritis patients, and 32 healthy individuals. Results: HER2/HBB and MYC/HBB ratios in tissue and cell-free plasma from gastric cancer patients were significantly higher than those of gastritis tissue and cancer-free individuals. An optimized cut-off value of plasma target gene to HBB ratio, used to differentiate cancer patients from cancer-free individuals, was evaluated using receiver operating characteristic (ROC) curves. Values of 2.0 were calculated for HER2 [area under the ROC curve (AUC), 0.760] and 2.725 for MYC (AUC, 0.767). A combination model of HER2 and MYC provided a better differentiation condition than that for HER2 or MYC only (AUC, 0.850). HER2/HBB ratios in plasma from gastric cancer patients correlated with MYC/HBB ratios. Conclusions: Our findings suggest that the measurement of plasma HER2 and MYC gene levels could improve the screening of gastric cancer.
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