Optimization of submerged culture conditions for the mycelial growth and exo-biopolymer production by Cordyceps militaris

Abstract

Aims: The objective of the present study was to determine the optimal culture conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture. Methods and Results: The optimal temperature and initial pH for both mycelial growth and exo-biopolymer production by Cordyceps militaris in shake flask culture were found to be 20°C and 6.0, respectively. Sucrose (40 g 1-1) and corn steep powder (10 g 1-1) were the most suitable carbon and nitrogen source for both mycelial growth and exo-biopolymer production. Conclusions: Under optimal culture conditions, the maximum exo-biopolymer concentration in a 5-1 jar fermenter indicated 10.3 g 1-1, which was approximately three times higher than that in shake flask culture. Significance and Impact of the Study: This process can have a significant impact on the industrial scale when sucrose and corn steep powder were used as carbon and nitrogen source.