Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth
- Authors
- Bak, Dong Ho; Choi, Mi Ji; Kim, Soon Re; Lee, Byung Chul; Kim, Jae Min; Jeon, Eun Su; Oh, Wonil; Lim, Ee Seok; Park, Byung Cheol; Kim, Moo Joong; Na, Jungtae; Kim, Beom Joon
- Issue Date
- Sep-2018
- Publisher
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Keywords
- Alopecia; Dermal papilla cell; Hair growth; IGFBP-1; Stem cell; Stem-cell therapy
- Citation
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, v.22, no.5, pp 555 - 566
- Pages
- 12
- Journal Title
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Volume
- 22
- Number
- 5
- Start Page
- 555
- End Page
- 566
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/815
- DOI
- 10.4196/kjpp.2018.22.5.555
- ISSN
- 1226-4512
2093-3827
- Abstract
- Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) are used in tissue repair and regeneration; however, the mechanisms involved are not well understood. We investigated the hair growth-promoting effects of hUCB-MSCs treatment to determine whether hUCB-MSCs enhance the promotion of hair growth. Furthermore, we attempted to identify the factors responsible for hair growth. The effects of hUCB-MSCs on hair growth were investigated in vivo, and hUCB-MSCs advanced anagen onset and hair follicle neogeneration. We found that hUCB-MSCs co-culture increased the viability and up-regulated hair induction-related proteins of human dermal papilla cells (hDPCs) in vitro. A growth factor antibody array revealed that secretory factors from hUCB-MSCs are related to hair growth. Insulin-like growth factor binding protein-1 (IGFBP-1) and vascular endothelial growth factor (VEGF) were increased in co-culture medium. Finally, we found that IGFBP-1, through the co-localization of an IGF-1 and IGFBP-1, had positive effects on cell viability; VEGF secretion; expression of alkaline phosphatase (ALP), CD133, and beta-catenin; and formation of hDPCs 3D spheroids. Taken together, these data suggest that hUCB-MSCs promote hair growth via a paracrine mechanism.
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