Antioxidative and Antitumor Effects of Isoflavones Isolated from the Leaves of Maackia fauriei.
- Authors
- Yoon, Ki Hoon; Park, Kwang Jun; Yin, Jun; Yoon, Kyu Hyung; Lee, Ji Yeon; Hwang, Yoon Jeong; Lee, Do Ik; Choi, Young Wook; Lee, Min Won
- Issue Date
- 2016
- Publisher
- ACG PUBLICATIONS
- Keywords
- Maackia fauriei; flavonoids; pterocarpans; anti-oxidant; anti-tumor; cytotoxicity
- Citation
- RECORDS OF NATURAL PRODUCTS, v.10, no.4, pp 441 - 451
- Pages
- 11
- Journal Title
- RECORDS OF NATURAL PRODUCTS
- Volume
- 10
- Number
- 4
- Start Page
- 441
- End Page
- 451
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/8676
- ISSN
- 1307-6167
- Abstract
- The flowers of Maackia fauriei have traditionally been used to treat hypertension, apoplexy, hemostasis, vaginal bleeding, and dystocia; moreover, the bark of this plant has been used as a natural dye. In the present study, activity-guided isolation of the leaves of M. fauriei yielded five isoflavones [genistein (1), pratensein (2), genistin (3), 2'-hydroxygenistein-7-O-beta-D-glucopyranoside (4), and 2,3-dehydrokievitone (5)]; three pterocarpans [medicarpin (6), maackiain (7), and 4-hydroxy maackiain (8)]; and one flavonol [isoquercitrin (9)]. To evaluate the anti-oxidative effects of these compounds, their 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assays and nitrotetrazolium blue chloride (NBT) superoxide scavenging assays were measured. And the anti-tumor activity against human cancer cell lines in genital system, LNCaP, PC-3, HeLa and OVCAR-3 cells were evaluated by MTT method. Furthermore, the apoptosis of the PC-3 and HeLa cells were determined by by annexin V-FITC and PI their fluorescence was analyzed by flow cytometry. The flavonol (9, isoquercitrin) and pterocarpan (8, 4-hydroxymaackiain) showed strong anti-oxidative activities. Besides, the isoflavones (1-5) did not showed anti-oxidative activity and the isoflavones (1-5) and pterocarpans (6-8) generally showed the potent cytotoxic activity against all of four human genital cancer cells. Especially, 2,3-dehydrokievitone (5) which had a prenyl group at C-8 position of the A-ring exhibited strong cytotoxic activity and induced apoptosis efficiently in cancer cells. (C) 2016 ACG Publications. All rights reserved.
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