Simple detection of residual enrofloxacin in meat products using microparticles and biochips
- Authors
- Ha, Mi-Sun; Chung, Myung-Sub; Bae, Dong-Ho
- Issue Date
- 2016
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Enrofloxacin; antibody; biochips; microparticles
- Citation
- FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, v.33, no.5, pp 817 - 823
- Pages
- 7
- Journal Title
- FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
- Volume
- 33
- Number
- 5
- Start Page
- 817
- End Page
- 823
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/8712
- DOI
- 10.1080/19440049.2016.1179560
- ISSN
- 1944-0049
1944-0057
- Abstract
- A simple and sensitive method for detecting enrofloxacin, a major veterinary fluoroquinolone, was developed. Monoclonal antibody specific for enrofloxacin was immobilised on a chip and fluorescent dye-labelled microparticles were covalently bound to the enrofloxacin molecules. Enrofloxacin in solution competes with the microparticle-immobilised enrofloxacin (enroMPs) to bind to the antibody on the chip. The presence of enrofloxacin was verified by detecting the fluorescence of enrofloxacin-bound microparticles. Under optimum conditions, a high dynamic range was achieved at enrofloxacin concentrations ranging from 1 to 1000 mu g kg(-1). The limits of detection and quantification for standard solutions were 5 and 20 mu g kg(-1) respectively, which are markedly lower than the maximum residue limit. Using simple extraction methods, recoveries from fortified beef, pork and chicken samples were 43.4-62.3%. This novel method also enabled approximate quantification of enrofloxacin concentration: the enroMP signal intensity decreased with increasing enrofloxacin concentration. Because of its sensitivity, specificity, simplicity and rapidity, the method described herein will facilitate the detection and approximate quantification of enrofloxacin residues in foods in a high-throughput manner.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Biotechnology & Natural Resource > School of Food Science and Technology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.