Y-27632, a Rho-Associated Protein Kinase Inhibitor, Inhibits Voltage-Dependent K plus Channels in Rabbit Coronary Arterial Smooth Muscle Cells
- Authors
- Li, Hongliang; Shin, Sung Eun; Kim, Hye Won; Kim, Han Sol; Jung, Won-Kyo; Ha, Kwon-Soo; Han, Eun-Taek; Hong, Seok-Ho; Choi, Il-Whan; Bae, Young Min; Firth, Amy L.; Bang, Hyoweon; Park, Won Sun
- Issue Date
- Dec-2016
- Publisher
- KARGER
- Keywords
- Y-27632; Rho-associated protein kinase; Voltage-dependent K plus channel; Coronary arterial smooth muscle cells
- Citation
- PHARMACOLOGY, v.98, no.5-6, pp 220 - 227
- Pages
- 8
- Journal Title
- PHARMACOLOGY
- Volume
- 98
- Number
- 5-6
- Start Page
- 220
- End Page
- 227
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/8766
- DOI
- 10.1159/000447745
- ISSN
- 0031-7012
1423-0313
- Abstract
- We examined the effects of the Rho-associated protein kinase (ROCK) inhibitor Y-27632 on voltage-dependent K+ (Kv) channels in rabbit coronary arterial smooth muscle cells using the whole-cell patch clamp technique. Y-27632 reduced the amplitude of the Kv current in a concentration-dependent manner, with an IC50 of 0.87 +/- 0.06 mu mol/l and a Hill coefficient of 1.48 +/- 0.06. Y-27632 did not affect the steady-state activation or inactivation curves, suggesting that the drug does not affect the voltage sensitivity of Kv channels. Another ROCK inhibitor, H-1152, did not affect the Kv current and had no significant effect on the Y-27632-induced inhibition of Kv channels, indicating that the inhibitory effect of Y-27632 on the Kv current is independent of ROCK signaling. From these results, we conclude that Y-27632 inhibits the Kv channel current in a dose-dependent and ROCK signaling-independent manner. (C) 2016 S. Karger AG, Basel
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Collections - College of Medicine > College of Medicine > 1. Journal Articles
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