3,4,5-Tricaffeoylquinic Acid Attenuates TRAIL-induced Apoptosis in Human Keratinocytes by Suppressing Apoptosis-related Protein Activation
- Authors
- Lee, Da Hee; Nam, Yoon Jeong; Lee, Min Sung; Sohn, Dong Suep; Shin, Yong Kyoo; Lee, Chung Soo
- Issue Date
- Oct-2015
- Publisher
- WILEY
- Keywords
- TRAIL; 3, 4, 5-tricaffeoylquinic acid; human keratinocytes; apoptosis-related protein activation; skin diseases
- Citation
- PHYTOTHERAPY RESEARCH, v.29, no.10, pp 1549 - 1558
- Pages
- 10
- Journal Title
- PHYTOTHERAPY RESEARCH
- Volume
- 29
- Number
- 10
- Start Page
- 1549
- End Page
- 1558
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9041
- DOI
- 10.1002/ptr.5425
- ISSN
- 0951-418X
1099-1573
- Abstract
- Caffeoyl derivatives exhibit antiinflammatory and antioxidant effects. However, the effect of 3,4,5-tricaffeoylquinic acid on the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in keratinocytes that may be involved in skin diseases has not been studied. In this respect, we investigated the effect of 3,4,5-tricaffeoylquinic acid on TRAIL-induced apoptosis in human keratinocytes. 3,4,5-Tricaffeoylquinic acid and oxidant scavengers attenuated the decrease in the cytosolic levels of Bid, Bcl-2, and survivin proteins; the increase in the levels of cytosolic Bax, p53, and phosphorylated p53; the increase in the levels of phosphorylated p38; the increase in the mitochondrial levels of the voltage-dependent anion channel; loss of the mitochondrial transmembrane potential; the release of cytochrome c; activation of caspases (8, 9, and 3); cleavage of poly [ADP-ribose] polymerase-1; production of reactive oxygen species; the depletion of glutathione (GSH); nuclear damage; and cell death in keratinocytes treated with TRAIL. These results suggest that 3,4,5-tricaffeoylquinic acid may reduce TRAIL-induced apoptosis in human keratinocytes by suppressing the activation of the caspase-8 and Bid pathways and the mitochondria-mediated cell death pathway. The effect appears to be associated with the inhibitory effect on the production of reactive oxygen species and depletion of GSH. 3,4,5-Tricaffeoylquinic acid appears to be effective in the prevention of TRAIL-induced apoptosis-mediated skin diseases. Copyright (c) 2015 John Wiley & Sons, Ltd.
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