Lipoteichoic acid upregulates NF-kappa B and proinflammatory cytokines by modulating beta-catenin in bronchial epithelial cellsopen access
- Authors
- Jang, Jaewoong; Kim, Wooyong; Kim, Kijeong; Chung, Sang-In; Shim, Yae Jie; Kim, Seok-Min; Yoon, Yoosik
- Issue Date
- Sep-2015
- Publisher
- SPANDIDOS PUBL LTD
- Keywords
- lipoteichoic acid; beta-catenin; bronchial epithelial cells; NF-kappa B; cytokine; inflammation
- Citation
- MOLECULAR MEDICINE REPORTS, v.12, no.3, pp 4720 - 4726
- Pages
- 7
- Journal Title
- MOLECULAR MEDICINE REPORTS
- Volume
- 12
- Number
- 3
- Start Page
- 4720
- End Page
- 4726
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9157
- DOI
- 10.3892/mmr.2015.3965
- ISSN
- 1791-2997
1791-3004
- Abstract
- Lipoteichoic acid (LTA) is a major cell wall component and virulence factor of gram-positive bacteria. The present study investigated the LTA-induced inflammatory response of BEAS-2B human bronchial epithelial cells, and detected the expression levels of proinflammatory cytokines interleukin (IL)-6, IL-8, IL-1 beta, tumour necrosis factor-a and monocyte chemotactic protein-1, the upregulation of NF-kappa B, and the phosphorylation and degradation of I-kappa B. During the LTA-induced inflammatory response of the BEAS-2B human bronchial epithelial cells, the activity levels of the beta-catenin-dependent promoter, and the protein expression levels of beta-catenin were significantly upregulated, whereas beta-catenin phosphorylation and the expression levels of AXIN were significantly downregulated. Following knockdown of beta-catenin by small interfering (si) RNA transfection, both the LTA-induced protein expression levels of NF-kappa B and the LTA-induced activity levels of the NF-kappa B-dependent promoter were significantly reduced. Similarly, a marked reduction in I-kappa B phosphorylation and degradation was observed following beta-catenin knockdown. The expression levels of the LTA-induced proinflammatory cytokines were also significantly reduced following beta-catenin siRNA. These results suggest that beta-catenin has a significant role in the regulation of NF-kappa B activity and proinflammatory cytokine expression during the LTA-induced inflammatory response of bronchial epithelial cells.
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