Anti-Amyloidogenic Properties of Dryopteris Crassirhizoma Roots in Alzheimer's Disease Cellular Model

Abstract

The inhibitory effects of Dryopteris crassirhizoma roots on A production, which is one of the major causes of Alzheimer's disease, were investigated as they have not been studied at all. The methanol extract of D.crassirhizoma roots significantly reduced the production of A in Chinese Hamster Ovarian cells overexpressing amyloid precursor proteins (APPs). This reduction was more pronounced with the butanol layer of D.crassirhizoma roots, which is partitioned based on solvent polarity. In addition, reduced production of A by D.crassirhizoma roots was accompanied with increased production of sAPP, which is a proteolytic fragment of APP by -secretases. The expression of -secretases was also decreased by treatment with D.crassirhizoma roots. Furthermore, the amount of CTF, a proteolytic fragment of APP by -secretases, is significantly increased by D.crassirhizoma roots. These results indicate that D.crassirhizoma roots efficiently decreased A production by inhibiting -secretases and increasing -secretases. Practical ApplicationsDryopteris crassirhizoma roots efficiently reduced A production. Therefore, D.crassirhizoma has the potential to be developed into an anti-A agent, which may have beneficial effects for the prevention or treatment of Alzheimer's disease.