Immobilization effect of bone morphogenetic protein-2 on collagen membrane via photoreactive gelatin derivatives: Biocompatibility and preservability of osteoinductive activity
- Authors
- Heo, Yun; Kim, Eun-Hye; Lee, Hyung-Jae; Jeong, Jin-Hong; Pyun, Do-Gi; Jang, Eui-Chan; Kim, Gang-Un; Rhee, Sung-Min; Son, Tae-Il; Song, Kwang-Sup
- Issue Date
- Jun-2015
- Publisher
- SPRINGER
- Keywords
- azidophenyl gelatin; photo-immobilization; collagen membrane; sustained protein release; bone morphogenetic protein-2 (BMP-2)
- Citation
- MACROMOLECULAR RESEARCH, v.23, no.6, pp 525 - 530
- Pages
- 6
- Journal Title
- MACROMOLECULAR RESEARCH
- Volume
- 23
- Number
- 6
- Start Page
- 525
- End Page
- 530
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9521
- DOI
- 10.1007/s13233-015-3068-3
- ISSN
- 1598-5032
2092-7673
- Abstract
- To photo-immobilize bone morphogenetic protein-2 (BMP-2), UV light-reactive gelatin (azidophenyl gelatin, Az-gel), which is introduced with an N-(4-azidobenzoyloxy) succinimide-containing an azide group, was prepared as a carrier of BMP-2. Characterization of the photo-reactivity of Az-gel was evaluated by the curing ratio and it was micropatterned using a photomask. The cross-linking ratio and an enzyme-linked immunosorbent assay, enzymelinked immunospecific assay (ELISA)-based BMP-2-release test were used to determine the optimal density of Azgel (1, 3, and 5%) and UV treatment time (10, 30, 60 s) of the BMP-2 carrier in collagen matrix. The results showed that 5% Az-gel with 60 s of UV treatment was the most efficient condition for cross-linking. Cytotoxicity assays using the C2C12 mouse myoblast cell line showed that there were no cytotoxic effects of Az-gel. Alkaline phosphatase activity and calcium ion detection assays to evaluate the osteoinductive activities were conducted in assumed optimal condition of Az-gel (5% and 60 s UV treatment) 14 days post-treatment. In our study, the osteoinductive activities were the highest in cells treated with BMP-2 and the photo-reactive Az-gel carrier compared to BMP-2 alone or untreated control cells in collagen matrix at all time points (3, 7, and 14 days).
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
- College of Medicine > College of Medicine > 1. Journal Articles
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