Gene expression profiling of coelomic cells and discovery of immune-related genes in the earthworm, Eisenia andrei, using expressed sequence tagsopen access
- Authors
- Tak, Eun Sik; Cho, Sung-Jin; Park, Soon Cheol
- Issue Date
- Mar-2015
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Coelomic cells; Earthworm; Expressed sequence tags; Innate immunity; Lysenin-related proteins
- Citation
- BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.79, no.3, pp 367 - 373
- Pages
- 7
- Journal Title
- BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- Volume
- 79
- Number
- 3
- Start Page
- 367
- End Page
- 373
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9763
- DOI
- 10.1080/09168451.2014.988677
- ISSN
- 0916-8451
1347-6947
- Abstract
- The coelomic cells of the earthworm consist of leukocytes, chlorogocytes, and coelomocytes, which play an important role in innate immunity reactions. To gain insight into the expression profiles of coelomic cells of the earthworm, Eisenia andrei, we analyzed 1151 expressed sequence tags (ESTs) derived from the cDNA library of the coelomic cells. Among the 1151 ESTs analyzed, 493 ESTs (42.8%) showed a significant similarity to known genes and represented 164 unique genes, of which 93 ESTs were singletons and 71 ESTs manifested as two or more ESTs. From the 164 unique genes sequenced, we found 24 immune-related and cell defense genes. Furthermore, real-time PCR analysis showed that levels of lysenin-related proteins mRNA in coelomic cells of E. andrei were upregulated after the injection of Bacillus subtilis bacteria. This EST data-set would provide a valuable resource for future researches of earthworm immune system.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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