Clinicopathologic Significance of BRAF Mutation and Extracellular Signal Regulated Kinase 1/2 Expression in Patients With a Colorectal Adenocarcinomaopen access
- Authors
- Kim, Hyung Ook; Kim, Beom Gyu; Cha, Seong Jae; Park, Yong Gum; Lee, Tae Jin
- Issue Date
- Feb-2015
- Publisher
- KOREAN SOC COLOPROCTOLOGY
- Keywords
- BRAF; ERK1; ERK2; Colorectal neoplasms; Adenocarcinoma
- Citation
- ANNALS OF COLOPROCTOLOGY, v.31, no.1, pp 9 - 15
- Pages
- 7
- Journal Title
- ANNALS OF COLOPROCTOLOGY
- Volume
- 31
- Number
- 1
- Start Page
- 9
- End Page
- 15
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9884
- DOI
- 10.3393/ac.2015.31.1.9
- ISSN
- 2287-9714
2287-9722
- Abstract
- Purpose: BRAF mutation and expression of extracellular signal regulated kinase (ERK) are linked with colorectal carcinogenesis through the serrated pathway. BRAF and ERK1/2 play important roles in the activation of mitogen-activated protein (MAP) kinase signaling pathways. The present study investigated the clinicopathologic outcomes of BRAF mutation and ERK1/2 expression in patients with colorectal cancer (CRC) and the possibility of using them as prognostic indicators. Methods: Dual-priming oligonucleotide-based multiplex polymerase chain reaction for BRAF(V600E) mutation and immunohistochemical analysis of ERK1/2 were performed using 65 formalin-fixed, paraffin-embedded samples from patients with CRC. We analyzed the dependences of the clinicopathologic features on BRAF mutation and ERK1/2 expression. Results: Out of 65 samples from CRC patients, BRAF mutation was detected in 3 (4.6%). The 3 patients with BRAF mutation presented with T3 CRC with lymph node metastasis (stage III) showing moderately or poorly differentiated histology. ERK1 and ERK2 were positively detected in 73.8% and 15.4% of the patients with CRC, respectively. ERK1 expression was significantly correlated with lymph node metastasis (P = 0.049). ERK2 expression was significantly correlated with tumor emboli (P < 0.05), tumor invasion (P = 0.035), lymph node metastasis (P = 0.017), and stage (P = 0.02). Conclusion: BRAF mutation and ERK1/2 expression may be associated with advanced or more aggressive CRC. These molecular markers might play prognostic roles in CRC developed through the serrated pathway.
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